Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR.
نویسندگان
چکیده
The attributes of the PCR allowed implementation of an assay for specific detection of Piscirickettsia salmonis from a few microliters of fish serum. This opens the way to less invasive modes of sampling for this microbial pathogen in salmonids.
منابع مشابه
Development of polymerase chain reaction assays for detection, identification, and differentiation of Piscirickettsia salm onis
A nested polymerase chain reaction (PCR) was developed to detect genomic DNA of Piscirickettsia salmonis, the causative agent of an epizootic disease in salmonids. The nested PCR assay, which used general bacterial 16s rDNA primers in the flrst amplification reaction, and P salmonisspecif~c primers in a second reaction, allowed detection of less than 1 P salmonis tissue culture infect~ous dose ...
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Piscirickettsia salmonis is a facultative intracellular Gram-negative bacterium (Mauel, Ware & Smith 2008; Mikalsen et al. 2008) originally isolated from fish in Chile and constitutes one of the main problems in farmed salmonids and marine fish worldwide (see reviews by Almendras & Fuentealba 1997; Mauel & Miller 2002; Fryer & Hedrick 2003). This fastidious pathogen was first observed in southe...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 64 8 شماره
صفحات -
تاریخ انتشار 1998